中文无码一区二区不卡av-亚洲午夜性猛春交xxxx-中文精品久久久久人妻-欧美日产国产精选-亚洲精品国产综合久久一线

當(dāng)前位置:網(wǎng)站首頁技術(shù)文章 > 斑馬魚8羥基脫氧鳥苷(8-OHdG)ELISA試劑盒引用文獻(xiàn)

斑馬魚8羥基脫氧鳥苷(8-OHdG)ELISA試劑盒引用文獻(xiàn)

更新時間:2021-01-07 點(diǎn)擊量:965

【文獻(xiàn)標(biāo)題】Fluoxastrobin-induced effects on acute toxicity, development toxicity,oxidative stress, and DNA damage in Danio rerio embryos

【作者】Cheng Zhang,Jingwen Zhang,Lusheng Zhu,et.al

【作者單位】山東農(nóng)業(yè)大學(xué)(Shandong Agricultural University)

【文獻(xiàn)中引用產(chǎn)品】

斑馬魚8羥基脫氧鳥苷(8-OHdG)ELISA試劑盒

【關(guān)鍵詞】96 h LC50,Delayed hatching,Teratogenicity,8-OHdG,AO-EB staining,Apoptosis

【DOI】doi.org/10.1016/j.scitotenv.2020.137069

【影響因子(IF)】5.92

出版期刊】《Science of the Total Environment》

【產(chǎn)品原文引用】

2.7. Measurement of DNA damage induced by FLUO

To evaluate DNA damage to Danio rerio larvae induced by FLUO, 8-OHdG contents and apoptosis were monitored. The supernatant was prepared from the homogenates prepared in Section 2.5 after centrifugation at 12,857g and 4 °C for 10 min. The esultant solution was kept on ice during the entire procedure. A prior study (Guo et al., 2014)noted the significance of 8-OHdG in evaluating DNA damage. The 8-OHdH contents were monitored using an 8-OHdG ELISA kit (Hengyuan Biological Technology Co., Ltd., Shanghai, China) at 450 nm for 10 min (Multiskan MK3, Thermo Fisher Scientific, Massachusetts, USA) and in units of ng/g protein. The total protein contents were assessed as in Section 2.6.As per Sun et al. (2019) and Wang et al. (2018), apoptosis was monitored using acridine orange (AO)-ethidium bromide (EB) double dyeing kit (Solarbio Science & Technology Co., Beijing, China). At 96 hpf, ten larvae from each trial in Section 2.5 were rinsed twice with PBS (pH =7.4) and dyed with 20 μL of AO and EB working fluid (1/1, v/v) for 5 min and then rinsed twice with PBS. Tritricaine (MS-222) was used for 3 min to anesthetize larvae for better observation. Then, the apoptosis of larvae was observed and photographed under an inverted fluorescence microscope (xioVert. A1, Carl Zeiss AG, Oberkochen, Germany).

完整版PDF文獻(xiàn)請咨詢在線客服或者聯(lián)系我司業(yè)務(wù)員!

更多公司福利請關(guān)注“恒遠(yuǎn)生物”VX公眾號!

主站蜘蛛池模板: 国精产品源xzl仙踪林仙踪| 澳门久久| 97无码精品综合| 欧美肥妇多毛bbw| 中文字幕无码乱人伦免费| 亚洲a∨无码一区二区| 国产18禁黄网站免费观看| 久久人搡人人玩人妻精品| 97久久精品无码一区二区天美| 国产莉萝无码av在线播放| 亚洲永久精品ww47永久入口| 国产专业剧情av在线| 一个人看的www片免费高清视频| 国产精品亚洲综合色区韩国| 久久久久香蕉国产线看观看伊 | 天干天干夜啦天干天干国产| 成人无码男男gv在线观看网站| 日本japanese丰满多毛| 精品国产丝袜黑色高跟鞋| 琪琪午夜理论片福利在线观看| 青青草国产午夜精品| 香港三日本三级少妇三级视频| а√天堂资源8在线官网在线| 欧美506070老妇乱子伦| 无遮挡高潮国产免费观看| 18禁成人???猛撞视频在线看| 女人自慰疯狂喷水| 中文字幕乱码亚洲影视| 无码人妻在线一区二区三区免费 | 国产乱码一区二区三区爽爽爽| 成人国产片视频在线观看| 精品无人区卡卡二卡三乱码| 在线看片免费人成视频福利 | 中文字幕 亚洲 无码 在线| 亚洲国产一区二区精品无码| 中文字幕丰满乱子无码视频| av无码动漫一区二区三区精品| 亚洲伊人色综合www962| 中文无码人妻有码人妻中文字幕| 亚洲免费观看在线视频| 国产av无码精品色午夜|